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乳清蛋白气凝胶的改性及对其装载性能的影响

Modification of whey protein aerogel and the influence on its loading performance

中文摘要英文摘要

【目的】冷冻干燥制得的乳清蛋白气凝胶抗压能力差,容易破损。为了增强乳清蛋白气凝胶的抗压性能,提高乳清蛋白气凝胶作为活性物质载体的适用性。【方法】用微晶纤维素和谷氨酰胺转氨酶改性乳清蛋白气凝胶。并比较改性前后气凝胶表面形貌、吸湿性、对亚油酸的装载率、保护性能及其在胃肠道中对亚油酸的释放曲线。【结果】改性后的乳清蛋白气凝胶有较好的抗压性能和活性物质装载率。SEM图像显示两种改性均能够提高乳清蛋白气凝胶孔径的均匀程度。其中,微晶纤维素改性后的气凝胶表面孔的孔径较大,而谷氨酰胺转氨酶改性气凝胶的表面形成的孔更小而均匀。上述气凝胶均能在肠道环境下选择性释放亚油酸。在高湿环境下,谷氨酰胺转氨酶改性的气凝胶吸湿能力最低,储存性更强,且对亚油酸的过氧化有更强的抑制作用。【结论】谷氨酰胺转氨酶改性乳清蛋白气凝胶,可提高其装载性能。

Objective] Whey protein aerogel prepared by freeze drying has low compressive strength and is easily broken. With the aim to improve the compressive properties of whey protein aerogel and to broaden its application as an active compounds carrier in the food industry.[Method] Whey protein aerogel was modified with microcrystalline cellulose and Glutamine transaminase. A comparative study of microstructural properties as well as moisture absorption of the resulting aerogels was carried out. The linoleic acid was loaded in three aerogels to evaluate their oil loading capacity, protective properties and release profile of linoleic acid in the simulation gastrointestinal tract.[Result] The modified whey protein aerogel exhibited better compressive strength and active compounds loading capacity. The voids at the microstructural images of two modified aerogels were more uniformly sized compared to the non-modified sample. MCC blending augmented the pores size of the aerogel, while the pores on the surface of the Glutamine transaminase-modified aerogel were smaller than the non-modified aerogel. The linoleic acid loaded in all three types of aerogels can be selectively released in the intestinal environment. The Glutamine transaminase-modified aerogel has the lowest moisture absorption under high humidity condition, and the stronger ability to decrease the loaded linoleic acid susceptibility to oxidation. [Conclusion] Glutamine transaminase-modified aerogel had a better loading performance.

卢蓉蓉、夏天利

生物工程学生物化学

食品生物技术乳清蛋白气凝胶微晶纤维素谷氨酰胺转氨酶活性物质载体

Food BiotechnologyWhey proteinAerogelMicrocrystalline CelluloseGlutamine transaminaseActive compounds carrier

卢蓉蓉,夏天利.乳清蛋白气凝胶的改性及对其装载性能的影响[EB/OL].(2017-12-22)[2025-08-10].http://www.paper.edu.cn/releasepaper/content/201712-289.点此复制

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