B16黑色素瘤、RM-1前列腺癌荷瘤小鼠的尿液蛋白质组研究

Urine can accumulate more changes and reflect early physiological and pathological changes of the disease, such as tumors. Therefore, urine is an ideal source for finding early biomarkers. In this study, melanoma and prostate cancer-bearing mouse models were established by subcutaneous injection of B16 and RM-1 cells, respectively. Urine samples were collected at four time points during tumor growth. Based on data-independent collection (DIA) technology, LC-MS / MS was used for quantitative analysis. Compared with that before the injection of B16 cells, 38 human homologous differential proteins were screened, of which 18 proteins were reported to be related to melanoma. Before the tumor was visible, there were four differential proteins and all were reported to be related to melanoma. Compared with that before the injection of RM-1 cells, a total of 14 human homologous differential proteins were screened, of which 9 proteins were reported to be associated with prostate cancer. Before the tumor was palpable, 9 proteins showed significant differences. There are significant differences between the two tumor-bearing models. Through the above experiments and analysis, the urine proteome can reflect the changes in the development and early biomarker information of the two tumors, and provide clues for the early clinical diagnosis of related tumors