左归丸对骨质疏松症大鼠GPR48、BSP表达影响的实验研究

Objective 　In order to explore the etiological factors and pathologh of the osteoporosis due to kidney deficiency,We study the expression of GPR48、BSP in blood serum of the rats of osteoporosis due to kidney deficiency by using ovariectomy.To explore the adjustable function of Zuogui Pill and its regulating mechanism. Method 　 By using ovariectomy, we established the rat model of osteoporosis due to Kidney-deficiency. We treated the osteoporosis rat with Zuogui Pill for 12 weeks. Simultaneously，used GaiTianLi tablets, as positive control groups. And rats in the normal group and model group were not given any treatment. We used the ELISA method to investigate the expression of the GPR48、BSP in blood serum of the rats. Result ELISA detection：the GPR48、BSP existed in the normal blood serum. Comparing with the normal group and fake operational group，the level of the expression of the GPR48、BSP in blood serum of model group decreased evidently. After the precaution treatment with Zuogui Pill group and Gaitianli tablets group, The Zuogui Pill group can evidently increase the expression level of the GPR48、BSP in blood serum. Conclusion ①In normal rats，the blood serum can express the GPR48、BSP in the protein level. ②The changing of GPR48、BSP protein expression in the blood serum, may be one of the important occurring mechanism of the osteoporosis due to kidney deficiency. ③The experimental Chinese herbs,which have the function of tonifying kidney,can increase the level of the expression of the protein of GPR48、BSP in blood serum. It would be preventing and treating to the osteoporosis due to kidney deficiency.