跨物种筛选中国特有珍稀濒危雉类-白冠长尾雉(Syrmaticus reevesii)10个微卫星位点
ross-species microsatellites amplification for a vulnerable species endemic of China, Reeves’s pheasant (Syrmaticus reevesii)
由于微卫星位点侧翼序列在一些鸟类中的相对保守性,使得跨物种筛选微卫星位点成为可能。白冠长尾雉(Syrmaticus reevesii)隶属于鸡形目(Galliformes)雉科(Phasianidae)长尾雉属(Syrmaticus),为我国II级重点保护动物和IUCN易危物种。为开展该物种的保护遗传学研究,采用跨物种筛选微卫星引物的方法,获得白冠长尾雉多态微卫星位点10个。等位基因数目从2到10不等,其期望杂合度在0.32到0.802之间。这些跨物种筛选引物与以往磁珠富集文库筛选的引物一起,将为白冠长尾雉相关保护遗传学研究提供重要手段
ross-species microsatellite amplification can be an effective way in obtaining microsatellite loci for closely related taxa in bird species. The Reeves’s pheasant, Syrmaticus reevesii, is a vulnerable species endemic of China. In order to study the population genetics and paternity of its wild populations, we obtained 10 polymorphic microsatellite markers from cross-species amplification. The number of alleles per locus varies between two and twelve, with expected heterozygosity ranging from 0.32 to 0.802 (n =75). These polymorphic loci are facilitating the study of paternity identification of Reeves’s pheasant and also provide a valuable tool for future studies on conservation genetics of this species.
孙全辉、张正旺、常江、古浪屿、王宁
遗传学动物学
白冠长尾雉微卫星位点引物跨物种筛选
Syrmaticus reevesiimicrosatelliteprimerscross-species amplification
孙全辉,张正旺,常江,古浪屿,王宁.跨物种筛选中国特有珍稀濒危雉类-白冠长尾雉(Syrmaticus reevesii)10个微卫星位点[EB/OL].(2009-02-25)[2025-08-10].http://www.paper.edu.cn/releasepaper/content/200902-1330.点此复制
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