Vip3A基因植物表达载体的构建及烟草的遗传转化
onstruction of plant expression vectors of Vip3A gene and transformation in tobacco
为了研究Vip3A基因在转基因抗虫植物中的应用，利用PCR技术克隆了苏云芽孢杆菌的Vip3A基因和烟草的EF1α启动子，以pBI121质粒为基本载体，构建了分别由组成型CaMV35S启动子和花特异表达的EF1α启动子驱动Vip3A基因的植物表达载体pBIVip3A和pBIEFVip3A，并通过农杆菌介导的方法对烟草进行了遗传转化。经PCR检测，外源基因已整合到烟草基因组中。
In order to study the application of Vip3A gene in transgenic insect–resistant plants，Vip3A gene of Baciius thuringiensis and EF1α promoter of tobacco were cloned by PCR.Two plant expression vectors pBIVip3A and pBIEFVip3A were constructed,in which the Vip3A gene driven by the constitutive promoter CaMV35S and petal tissue–specific promoter EF1α respectively,and tobaccos were tansformed by Agrobacterium containing the constructed vectors.The transgenic lines were selected by PCR detestion.
邓伟、杨迎伍、马作江、李正国
遗传学生物工程学植物学
Vip3A基因EF1α启动子苏云金杆菌载体构建烟草
Vip3A gene EF1α promoter Bacillus thuringiensis construction of expression vector tobacco
邓伟,杨迎伍,马作江,李正国.Vip3A基因植物表达载体的构建及烟草的遗传转化[EB/OL].(2008-03-04)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/200803-68.点此复制
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