蜂毒对胶原诱导性关节炎炎性痛大鼠背根神经节TrkA、TRPV1的影响
目的 探讨蜂毒对胶原诱导性关节炎（collagen-induced arthritis, CIA）大鼠TrkA、TRPV1疼痛信号分子的影响。方法 分为正常对照组、模型组、蜂毒组（BV, 3 mg/mL）。采用Wistar雄性成年大鼠，Collagen Ⅱ+IFA 0.2 mL造模。BV组于造模14 d后开始给药，直至21 d；记录每组足跖厚度、痛阈和肿胀关节评分，背根神经节采用IHC观察病理切片TRPV1的表达和westernboltting观察TrkA的表达，评价蜂毒对CIA大鼠踝关节的干预。 结果 造模从10 d起开始肿胀，14 d内全部出现CIA体征，足跖厚度和肿胀关节评分整体上呈逐渐增加的趋势。在14 d时趋于平稳。BV组予蜂毒0.3 mg治疗7 d，BV组足跖厚度和肿胀关节评分低于模型组。痛阈（秒）：正常对照组15.47±0.35，模型组10.90±0.10，BV组13.14±0.18。免疫组化TRPV1阳性细胞百分数（%）：正常对照组 11.40±1.48，模型组 44.47±4.38，BV组 21.60±2.20。western blotting 观察 TrkA表达（灰度值）：正常对照组1.59±0.04，模型组4.53±0.21，BV组2.46±0.17。结论 蜂毒注射能降低炎性痛大鼠DRG信号分子TrkA、TRPV1的表达水平。这可能是消炎镇痛的信号通路之一，为蜂毒治疗类风湿性关节炎提供新的启发。
Objective To investigate the therapeutic effect of acupoint injection of bee venom on collagen-induced arthritis (CIA) in rats and explore the mechanism of bee venom therapy in the treatment of rheumatoid arthritis. Methods Fifteen male Wistar rats were randomly divided into bee venom treatment group (BV group), CIA model group, and control group. In the former two groups, CIA was induced by injections of collagen II + IFA (0.2 mL) via the tail vein, and in the control group, normal saline was injected instead. The rats in BV group received daily injection of 0.1 mL (3 mg/mL) bee venom for 7 consecutive days. All the rats were assessed for paw thickness and arthritis index from days 14 to 21, and the pain threshold was determined on day 21. The expressions of TRPV1 and TrkA in the dorsal root ganglion at the level of L4-6 were detected using immunohistochemistry and Western blotting, respectively. Results The rats in CIA model group started to show paw swelling on day 10, and by day 14, all the rats in this group showed typical signs of CIA. In BV group, the rats receiving been venom therapy for 7 days showed a significantly smaller paw thickness and a low arthritis index than those in the model group. The pain threshold was the highest in the control group and the lowest in the model group. TRPV1-positive cells and TrkA expression in the dorsal root ganglion was significantly reduced in BV group as compared with that in the model group. Conclusions Injection of bee venom can decrease expression of TRPV1 and TrkA in the dorsal root ganglion to produce anti-inflammatory and analgesic effects, suggesting the potential value of bee venom in the treatment of rheumatoid rthritis.
冼培凤、杨路、王升旭、彭澎、刘国涛、陈莹
基础医学内科学
蜂毒辣椒素受体络氨酸激酶A类风湿关节炎胶原诱导性关节炎
冼培凤,杨路,王升旭,彭澎,刘国涛,陈莹.蜂毒对胶原诱导性关节炎炎性痛大鼠背根神经节TrkA、TRPV1的影响[EB/OL].(2017-12-27)[2025-08-16].https://chinaxiv.org/abs/201712.00937.点此复制
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