干酪乳杆菌JH23代谢产物对单胺氧化酶的抑制作用

mong the tested Lactobacillus strains assayed for Inhibitory activity on monoamine oxidase (MAO), the supernatant of Lactobacillus casei JH23 cultured in MRS broth showed strong inhibitory activity. The bioactive components from the supernatant could be fractioned into organic solvent soluble (O) and water soluble ones (W) by extraction with ethyl acetate. Both the organic solvent soluble (O) and water soluble components (W) demonstrated a competitive inhibitory activity to MAO in a dose-dependent manner. The IC50 for the organic solvent soluble components (O) to MAO-A and MAO-B were 1.97mg / ml, 5.67mg / ml, while IC50 for the water soluble components (W) were 1.59mg / ml and 4.83mg / ml respectively. A bioactive fraction w1 was obtained from the water soluble components (W) by sephadex-G15 chromatograph, which the IC50 for MAO-A and MAO-B were 0.327mg/ml and1.13mg/ml .W1 could be further fractioned into 2 peaks with absorbance at 200nm and 300nm respectively by RP-HPLC, and the peak appeared at 200nm had good MAO inhibitory effect. The substance will be prepared by liquid phase and determined structure, which can promote the development of spiritual degeneration disease drug.