Specificity of SARS-CoV-2 antibody-detection assays against S and N protein among pre-COVID-19 sera from patients with protozoan and helminth parasitic infections
Specificity of SARS-CoV-2 antibody-detection assays against S and N protein among pre-COVID-19 sera from patients with protozoan and helminth parasitic infections
ABSTRACT BackgroundWe aimed to assess the specificity of SARS-CoV-2 antibody detection assays among people with known tissue-borne parasitic infections. MethodsWe tested three SARS-CoV-2 antibody-detection assays (cPass SARS-CoV-2 Neutralization Antibody Detection Kit, Abbott SARS-CoV-2 IgG assay, and STANDARD Q COVID-19 IgM/IgG Combo Rapid Test) among 559 pre-COVID-19 sera. ResultsThe specificity of assays was 95-98% overall. However, lower specificity was observed among sera from patients with protozoan infections of the reticuloendothelial system, such as human African trypanosomiasis (Abbott Architect; 88% [95%CI 75-95]), visceral leishmaniasis (SD RDT IgG; 80% [95%CI 30-99]), and from patients with recent malaria from a holoendemic area of Senegal (ranging from 91% for Abbott Architect and SD RDT IgM to 98-99% for cPass and SD RDT IgG). For specimens from patients with evidence of past or present helminth infection overall, test specificity estimates were all ≥ 96%. Sera collected from patients clinically suspected of parasitic infections that tested negative for these infections yielded a specificity of 98-100%. The majority (>85%) of false-positive results were positive by only one assay. ConclusionsThe specificity of SARS-CoV-2 serological assays among sera from patients with tissue-borne parasitic infections was below the threshold required for decisions about individual patient care. Specificity is markedly increased by the use of confirmatory testing with a second assay. Finally, the SD RDT IgG proved similarly specific to laboratory-based assays and provides an option in low-resource settings when detection of anti-SARS-CoV-2 IgG is indicated.
Corsini Rachel、Ndiaye Magatte、Zaharatos Gerasimos、B¨1scher Philippe、Ndao Momar、Camargo Fabio Vasquez、Harrison Luke B、Papenburg Jesse、Yansouni Cedric P、Faye Babacar、Caya Chelsea、Cheng Matthew P.、Matlashewski Greg
McGill Interdisciplinary Initiative in Infection and ImmunityFaculty of Medicine, Department of Parasitology, University of Cheikh Anta DiopDivision of Microbiology, Department of Clinical Laboratory Medicine, Optilab Montreal - McGill University Health CentreDepartment of Biomedical Sciences, Institute of Tropical MedicineDivision of Infectious Diseases, Department of Medicine, McGill University Health Centre||McGill Interdisciplinary Initiative in Infection and Immunity||National Reference Centre for Parasitology, Research Institute of the McGill University Health Centre||Department of Microbiology and Immunology, McGill UniversityNational Reference Centre for Parasitology, Research Institute of the McGill University Health CentreDivision of Infectious Diseases, Department of Medicine, McGill University Health Centre||Division of Microbiology, Department of Clinical Laboratory Medicine, Optilab Montreal - McGill University Health CentreDivision of Microbiology, Department of Clinical Laboratory Medicine, Optilab Montreal - McGill University Health Centre||McGill Interdisciplinary Initiative in Infection and Immunity||Division of Pediatric Infectious Diseases, Department of Pediatrics, Montreal Children?ˉs Hospital||Department of Epidemiology, Biostatistics, and Occupational Health, School of Population and Global Health, McGill UniversityJ.D. MacLean Centre for Tropical Diseases, McGill University||Division of Infectious Diseases, Department of Medicine, McGill University Health Centre||Division of Microbiology, Department of Clinical Laboratory Medicine, Optilab Montreal - McGill University Health Centre||McGill Interdisciplinary Initiative in Infection and ImmunityFaculty of Medicine, Department of Parasitology, University of Cheikh Anta DiopMcGill Interdisciplinary Initiative in Infection and ImmunityDivision of Infectious Diseases, Department of Medicine, McGill University Health Centre||Division of Microbiology, Department of Clinical Laboratory Medicine, Optilab Montreal - McGill University Health Centre||McGill Interdisciplinary Initiative in Infection and ImmunityDepartment of Microbiology and Immunology, McGill University
医药卫生理论医学研究方法基础医学
“SARS-CoV-2”“COVID-19”“Diagnostic accuracy”“Antibody test”“Serology”“Parasitic infections”“Malaria”“Kinetoplastid infections”“Protozoan infections”“Helminth infections”“Strongyloides”“Schistosoma”“Filaria”“Trichinella”“Neglected tropical diseases”
Corsini Rachel,Ndiaye Magatte,Zaharatos Gerasimos,B¨1scher Philippe,Ndao Momar,Camargo Fabio Vasquez,Harrison Luke B,Papenburg Jesse,Yansouni Cedric P,Faye Babacar,Caya Chelsea,Cheng Matthew P.,Matlashewski Greg.Specificity of SARS-CoV-2 antibody-detection assays against S and N protein among pre-COVID-19 sera from patients with protozoan and helminth parasitic infections[EB/OL].(2025-03-28)[2025-04-28].https://www.medrxiv.org/content/10.1101/2021.08.10.21261841.点此复制
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