益气活血药对人胚肺成纤维细胞增殖及胶原合成的影响
Effects of Supplementing Qi and Activating Blood Decoction on Proliferation and collagen Synthesis of MRC-5
目的：研究活血化瘀药川芎、丹参、莪术、桃仁与补气药黄芪配伍防治肺纤维化的细胞生物学效应及其作用机制。方法：将中药饮片水煎液浓缩至1g/ml，配成0.1%、1%、10%三个浓度干预MRC-5细胞，用MTT比色法检测对细胞活性和增殖的影响，天狼星红染色法观察药物对胶原沉积的作用。结论：川芎和莪术对MRC-5细胞的增殖、细胞活性和胶原沉积具有抑制作用，并呈浓度依赖性；而丹参的抑制作用为非浓度依赖；与黄芪配伍，能够拮抗川芎对MRC-5细胞的抑制作用。
Objective: To explore the anti-fibrosis effects and mechanisms of Ligusticum wallichii, Salvia miltiorrhiza, Rhizoma curcumae, Peach kernel and Astragalus propinquus. Method: MRC-5 cells were treated with different concentrations of decoction of these five decoction pieces and their combinations. MTT was employed to study the cytotoxicity and proliferalion.The collagen accumulation was determined by the optical density at 540nm of picrosirius red staining. Result: Ligusticum wallichii and Rhizoma curcumae could inhibit proliferation and collagen synthesis. Forthemore the cytotoxicity and inhibition rate of proliferation and collagen synthesis were concentration-dependence but Salvia miltiorrhiza was not.The inhibition of Ligusticum wallichii was induced by the combination with Astragalus propinquus.
赵丕文、田如玉、李亚东、马雪曼、牛建昭、亚晓旭、马雷雷、李彧
中医学基础医学细胞生物学
纤维化活血化瘀成纤维细胞黄芪
fibrosissupplementing qi and activating bloodfibroblaststragalus propinquus
赵丕文,田如玉,李亚东,马雪曼,牛建昭,亚晓旭,马雷雷,李彧.益气活血药对人胚肺成纤维细胞增殖及胶原合成的影响[EB/OL].(2012-02-28)[2025-08-18].http://www.paper.edu.cn/releasepaper/content/201202-1072.点此复制
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