代谢组学在肺癌诊断中的应用研究

Objectives:The UPLC/Q-TOF metabonomics analysis methods is applied to screen tumor biomarkers in lung cancer.Methods:24 cases of healthy people and 32 cases of three different pathological types of lung cancer patients's plasma were collected. Plasma was excepted protein. With ultra-high performance liquid chromatography/ mass-spectrometry(UPLC/Q-TOF) for chromatographic separation, mass spectrometry detection and data acquisition. Data-processing was norma- lized used markerlynx software. The data was imported to SIMCA-P+ software for denoising. Finally, partial least squares (PLS) was employed for analysis.Results: 24 cases of healthy patients and 32 cases of lung cancer were distinguish well by metabolomics methods.On the pattern of ESI+,7 substances was found.on the pattern of ESI-,8 substances was found Conclusions:UPLC/Q-TOF metabonomics analysis methods may be used to identify lung cancer biomarkers.