间斑寇蛛粗毒的生理生化分析:两种采毒方法的比较

he bioactivities and proteinous compositions of venoms collected by two different methods from L. tredecimguttatus were analyzed and compared. Most of protein components of the venom were high-molecular-mass acidic proteins (>10 kDa). The content of proteins with molecular masses at around 100 kDa, in the venom obtained by electrical stimulation (ESV) were greater than the venom obtained from the dissected venom gland (DGV). And the proteins and peptides with low molecular mass (<10 kDa) in the two kinds of venoms were very similar. The LD50 values of ESV and DGV in mice were 0.16±0.03 mg/kg and 0.39±0.05 mg/kg, respectively. LD50 values of ESV and DGV in cockroaches were 1.87μg/g and 2.32μg/g, respectively. ESV was able to block neuromuscular transmission in the mouse phrenic nerve-hemidiaphragm preparation within 25.0±2.2 min at a concentration of 3.2×10-6 g/mL, whereas DGV blocked neuromuscular transmission within 45.7±1.8 min at the same concentration. The fraction of the venom containing proteins and peptides with molecular mass <10 kDa did not obviously affect the transmission. Those results demonstrated that L. tredecimguttatus venom was a mixture rich in larger proteins rather than smaller proteins and peptides; the mammalian toxicities of the venom were due primarily to the high molecular weight acidic proteins rather than low molecular weight peptides; the active components contained in ESV and DGV were similar but the content in ESV was higher than that in DGV.