FTO过表达对小鼠胰岛β细胞功能的影响以及全基因组表达谱变化研究

o study and explore the effect of FTO (fat mass and obesity associated) overexpression on mouse pancreatic islet β cells and genes expression profile, mouse FTO gene was cloned into the lentivirus. Lentivirus was packaged in 293T cells and delivered into the pancreatic islet cells. QPCR and Western blot assay were employed to detect the overexpression of FTO in MIN6 cells. Glucose-stimulated insulin secretion (GSIS) was performed to detect the insulin secretion of MIN6 cells. Mouse gene microarray was used to detect the differential gene expression profile of the islet cells modulated by FTO overexpression. MIN6 cells stably expressing FTO was achieved by lentivirus delivery. FTO overexpression significantly inhibits the insulin secretion. The microarray shows that there are totally 922 differential expression genes. FTO overexpression changes the gene expression profile of MIN6 cells. The differentially expressed genes may regulate the islet β cells functions via important signal pathways.