连作和头茬地黄根系钙离子浓度差异及钙信号抑制剂对连作缓解效应分析

alcium signals play important roles in plants in response to stress. Preliminarily studies have been indicated that calcium signals are involved in the response process that perceived and transduced harmfulness caused by consecutive monoculture practice. To identify roles of calcium signals in consecutive monoculture harmfulness, calcium ion concentration in the root cells of R.glutinosa were measured by using pyroantimonate precipitation and calcium fluorescent dyes. Simultaneously, calcium signal blockers were applied to its verification feasibility as agentia treated consecutive monoculture disease. As a result, consecutive monoculture stress significantly promoted the accumulation of intracellular calcium level in root tip and root cells of R.glutinosa. The heparin sodium had obvious effects for alleviating consecutive monoculture harmfulness compared to the untreated consecutive monoculture R.glutinosa. This study further proved roles of calcium signals in consecutive monoculture formation, and laid a soild foundation for thoroughgoing solving consecutive monoculture problems.?????