新型双光子激发/近红外发光的AIE探针用于细胞内Zn2+荧光成像
Novel Two-Photon Excitation/Red-Emission AIE Chemsensor for Zn2+ Imaging Assay in Living Cells
在本文中，我们合成了一种新型双光子激发/近红外发光的AIE（聚集诱导发光）荧光探针TPE-QB，用于水溶液和细胞内的锌离子检测。其作用原理是在新型近红外AIE荧光团上修饰的席夫碱基团能够与锌离子特异性络合，致使原本在水溶液中分散存在的TPE-QB聚集，从而发出很强的近红外荧光。TPE-QB的发射波长达到650 nm,对锌离子具有很好的选择性和很高的灵敏度。此外。它还具有很好的生物相容性，可用于细胞内锌离子单光子/双光子的检测成像和亚细胞水平的锌离子示踪。
In this paper，a novel two-proton excited fluorescent sensor for the detection of zinc ion in aqueous solution as well as in cells was synthesized on the basis of complexation process using an aggregation-induced emission fluorophore. The near-infrared chemosensor possessing a schiff base derivatives with salicylaldehyde moiety show a distinctly selective response to zinc ion. The probe (TPE-QB) based on tetraphenylethene structure complexed zinc ion and then aggregated in aqueous buffered solution, causing remarkable enhancement (OFF-On) of red emissions at 650 nm. Furthermore, this fluorescent molecule easily penetrated cytomembrane and successfully achieved intracellular zinc ion imaging in living cells
李祖豪、李继山
生物化学生物物理学细胞生物学
化学生物传感聚集诱导发光锌离子检测近红外探针双光子成像
Biochemical SensorAggregation induced emissionZn2+ MonitorNear-Infrared Emissive ChemsensorTwo-photon Imaging
李祖豪,李继山.新型双光子激发/近红外发光的AIE探针用于细胞内Zn2+荧光成像[EB/OL].(2019-05-16)[2025-08-16].http://www.paper.edu.cn/releasepaper/content/201905-173.点此复制
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