“双固一通”针法对家兔缺血心肌电活动及其超微结构的影响

Objective To research the therapeutic effects of Shuangguyitong therapy of electroacupuncture on action potentials and myocardial ultrastructures of experimental acute myocardial ischemia model in rabbits . Methods 30 rabbits were divided into the control group, model group, Shuangguyitong group. Electrocardiography(ECG), mean arterial pressure(MAP), transmembrane action potential(TAP) were examined in rabbits. Experimental acute myocardial ischemia model by ligation of anterior descending branch of the left coronary artery was induced in rabbits. The myocardial ultrastructure and ransmembrane potential were observed. Results The Shuangguyitong therapy of electroacupuncture has favorable regulative action on the electrical and mechanical activities of the ischemic heart, results indicated that electroacupunctrure could markedly promote recovery of ST segment of electrocardiography （P<0.05）and the mean arterial pressure after ischemia, inhibit reduction of transmembrane action potential amplitude(APA) （P<0.05） and accelerate restoration of transmembrane action potential durations (APD50 、 APD90) （P<0.05）. In the acupuncture group, less injury of myocardial tissure, normal run of myocardial fiber, a small amount mitochondrial vacuolation and no obvious dissolution and necrosis of myocardium were found. Conclusion The Shuangguyitong therapy of electroacupuncture can obvious reduce the degree of myocardial ischemia, and improve the ability of anti-myocardial ischemia on the rabbits. It is very important to strengthening Zhengqi during treatment .