木豆叶治疗骨坏死的实验研究

Objective：To observe the effect of Folium Cajani (FC) in the treatment of osteonecrosis, and analyze the possible mechanism. Methods: Rabbit osteonecrosis models were established, and randomly divided into 2 groups: the control group (n=10) and the experimental group (n=10). All rabbits of 2 groups were operated with core decompression, and infilled with tissue engineering bone (nano-Hydroxyapatite/Collagen loaded with mesenchymal stem cells) in osteonecrosis area. Beginning on the days of operation, the rabbits of experimental group were given FC (7.68g/kg), while the control group were given saline (20ml/kg) by gastric irrigation once a day. After operation 12 weeks, all rabbits were killed．The changes of serum calcium/phosphorus level and bone regeneration in osteonecrosis area were observed. Vascular endothelial growth factor (VEGF) and osteocalcin expression were also observed with immunohistochemical studies. Results: Repair of osteonecrosis in experimental group was obviously superior to control group. In experimental group, higher VEGF and osteocalcin expression level was observed than control group. Serum calcium/phosphorus level was also higher in experimental group than in control group. Conclusion: FC can repair osteonecrosis through the mechanism of improving vascular and bone regeneration.