复黄生肌愈创油膏对糖尿病模型创面TGF-β1、samd3、samd7水平的动态影响

Objective:To explorer dynamic effects of“Compound Rhubarb Granulation-Promoting and Wound-Healing Ointment”(CRGWO) on the expression of TGF-β1,samd3,samd7 of granulation tissue of Wister rat diabetic wound Healing. Methods: Thirty-six male Wister rats were randomized into 3 groups:diabetic trial (CRGWO) group, normal control group, diabetic control group. The expression of TGF-β1,samd3,samd7 of granulation tissue of Wister rat diabetic wound were detected by immunohistochemical method in day 3 and day 11. Results: On the third day of wound healing, the expression of TGF-β1 and smad3 in diabetic trial group were higher than that of diabetic control group (P<0.05), but compare to the normal control group without statistical difference; the expression of smad7 in three groups have not statistical difference. On the eleventh day of wound healing, the expression of TGF-β1 and smad3 in diabetic trial group were lower than those of normal control group (P<0.05),but compare to the diabetic control group have not statistical difference; the expression of smad7 in diabetic trial group were higher than those of the others groups (P<0.05).Conclusion:The expression of TGF-β1 and smad3 in diabetic trial group were ascending in the early phase of wound healing which shows the CRGWO can promote wound healing via spurring the expression of certain cytokines and their signal transduction; in the late phase of wound healing, the expression of TGF-β1 and smad3 in diabetic trail group were descending, as well as the expression of Smad7, –the inhibitor of Smads in cell signal transduction, were ascending, which means CRGWO may open the inverse signal feedback of wound healing, thus prevent the scars formation by stopping the excessive cells proliferating.