芸薹属植物游离小孢子培养诱导双单倍体研究进展

Using isolated microspore culture to induce doubled haploid (DH) in Brassica can accelerate the purification rate, exhibit stable recessive trait, and enrich the germplasm resources. So it is widely used in the study of genetic breeding and molecular biology. This paper comprehensively expounds influence of the internal factors --- the genotype, developmental period of microspore, the flowering time, and the external factors --- the growth environment of the donor plant, the pretreatment, the media, the environment of culture and chromosome doubling on microspore culture in Brassica, in the hope to provide reference for establishing an efficient microspore culture system in Brassica.