Synthesis of Proteins by Automated Flow Chemistry
Synthesis of Proteins by Automated Flow Chemistry
Ribosomes produce most proteins of living cells in seconds. Here we report highly efficient chemistry matched with an automated fast-flow instrument for the direct manufacturing of peptide chains up to 164 amino acids over 328 consecutive reactions. The machine is rapid - the peptide chain elongation is complete in hours. We demonstrate the utility of this approach by the chemical synthesis of nine different protein chains that represent enzymes, structural units, and regulatory factors. After purification and folding, the synthetic materials display biophysical and enzymatic properties comparable to the biologically expressed proteins. High-fidelity automated flow chemistry is an alternative for producing single-domain proteins without the ribosome.
Sarah Antilla、Stephanie Hanna、Andrei Loas、Alexander J. Mijalis、Anthony J. Quartararo、Shunying Liu、Bradley L. Pentelute、Zachary P. Gates、Alexander J. Callahan、Mackenzie Poskus、Nina Hartrampf、Mark D. Simon、Carsten Jessen、Azin Saebi、Amanda E. Cowfer、Carly K. Schissel、Thomas E. Nielsen、Xiyun Ye
Sarah AntillaStephanie HannaAndrei LoasAlexander J. MijalisAnthony J. QuartararoShunying LiuBradley L. PenteluteZachary P. GatesAlexander J. CallahanMackenzie PoskusNina HartrampfMark D. SimonCarsten JessenAzin SaebiAmanda E. CowferCarly K. SchisselThomas E. NielsenXiyun Ye
生物化学生物工程学生物科学研究方法、生物科学研究技术
Peptide synthesisprotein synthesisFlow chemistry
Sarah Antilla,Stephanie Hanna,Andrei Loas,Alexander J. Mijalis,Anthony J. Quartararo,Shunying Liu,Bradley L. Pentelute,Zachary P. Gates,Alexander J. Callahan,Mackenzie Poskus,Nina Hartrampf,Mark D. Simon,Carsten Jessen,Azin Saebi,Amanda E. Cowfer,Carly K. Schissel,Thomas E. Nielsen,Xiyun Ye.Synthesis of Proteins by Automated Flow Chemistry[EB/OL].(2020-02-12)[2025-06-25].https://chemrxiv.org/engage/chemrxiv/article-details/60c74805f96a00bcb1286ff5.点此复制
评论