硫酸化修饰对党参多糖体外抗Ⅰ型单纯疱疹病毒的影响

Objective: Sulfated modification was carried out for codonopsis pilosula polysaccharide (SCPPS-1) and evaluated the antiviral activitiy of SCPPS-1 on herpes simplex virus-1 (HSV-1). Method: Codonopsis pilosula polysaccharide was sulfated using a sulfamic acid method, and the ester sulfate structure was identified by Infrared spectrum, the ester sulfate level was turbidimetrically measured in barium chloride - gelatin. The inhibitory effect against HSV-1 were studied by MTT on Vero cell. Result: Infrared spectrum proved the ester sulfate of codonopsis pilosula polysaccharide hydroxyl portion was replaced by the sulfate ion, showed the sulfate ion of the characteristic absorption peak in 1290 cm-1 and 799 cm-1. The sulfuric acid sulfate substitution degree of D = 0.754. According to MTT assay, cell cytotoxic concentration of maximum non-toxic concentration (TC0) was calculated. For CPPS-1 and SCPPS-1, TC0 was 100μg/mL and 200μg/mL, respectively. The survival rates of vero were higher than control group, under two different administration (treatment to medicine group, preventive medicine group) of CPPS-1 by MTT assay, but lower than the ACV group. While under two different administration, the survival rates in SCCPPS-1 group were higher than control group（P<0.01）, its preventive effect was equal to ACV（P>0.05）, its therapeutic effect was better than that of ACV（P<0.05）. Conclusion: CPPS-1 had some certain preventive effect on HSV-1 infection but no therapeutic effect, while SCCPPS-1 showed strong prevention and therapeutic effect.