小半夏汤对化疗性异食癖大鼠P物质和NK1受体的影响

Objective: In order to explore the mechanism of Xiaobanxiatang (XBXT) on the prevention and treatment of chemotherapy-induced nausea and vomiting (CINV), the effect of XBXT on substance P and NK1 receptor in chemotherapy-induced pica in rats was observed. Methods: The chemotherapy rat pica model was established by i.p. injection of 6mg/kg cisplatin. The Wistar rats were randomly divided into normal control group, XBXT normal control group, cisplatin model group, positive drug ondansetron group, XBXT high and low dose groups. The rats in ondansetron group, XBXT normal control group, XBXT high and low dose groups were respectively given 2.6mg/kg/d ondansetron, 1.6, 3.2 and 1.6g/kg/d XBXT by oral gavage before modeling 1h and then twice a day. The rats in normal control group and model group were gavaged with equal volume of distilled water. Kaolin consumptions were weighed and calculated once per 12h. After modeling 24h and 72h, substance P contents in rat serum, ileum and medulla oblongata, and preprotachykinin A (PPTA) and NK1 receptor mRNA expression levels in ileum and medulla oblongata were measured. Results: Kaolin consumptions in model group were significantly increased. The high and low dosages of XBXT could significantly inhibit kaolin consumptions in cisplatin-treated rats, reduce substance P contents in serum, ileum and medulla oblongata, and inhibit the abnormal expression of PPTA and NK1 receptor mRNA expression in ileum and medulla oblongata. Conclusion: The mechanism of XBXT on the prevention and treatment of CINV was related to the decrease of substance P contents and down regulation of NK1 receptor mRNA expression induced by cisplatin.