人类胚胎干细胞向造血分化外环境的初步研究

omparing the different roles and gene expression profiles of the human fetal liver stroma cells and the human bone marrow stroma cells in the hematopoietic differentiation from the human embryonic stem cells, to get insight of the differentiation mechanism. 4th day EBs were cocultured with hFLSCs supplemented with cytokines or the hFBMSC with cytokines. The flow cytometric analysis was performed after 8 days induction to detect the cells with hemangioblastic marker. The results showed that there were 1.06% ± 0.20%、8.8% ± 1.49% KDR positive cells and 1.25% ± 0.16%、9.19% ± 2.10% CD34 positive cells in each groups respectively；and the hemagioblastic-like cell colonies were counted, 0.9 ± 0.36、10.6 ± 0.63 cell colonies were found in each group. The result indicating that coculture with hFBMSC could promote the hemangioblastic differentiation from the hESCs. Differential gene expression profile between hFBMSC and hFLSCs were presented by gene-chip analysis to elucidate the directed hemangioblatic differentiation mechanism. The results showed that 240 genes which were highly expressed in the hFBMSC and we focused on secreted cytokines, cell adhesion molecules and extracellular matrix proteins. 21 genes were found related to these categories, which might play an important role to make up the extramicroenvironment of the hemangioblastic differentiation in vitro. They could activat the signal path way such as smad, JAK-STAT, MAPK to regulate the hESCs differentiation.