非接触式高压静电场微胶囊制备方法研究

non-contacted device based on the principle of high potentiometric dispersion technology for cell microencapsulation is designed and manufactured. Sodium alginate is solidified in calcium chloride solution. Integrated microencapsules with smooth surface and even diameter are prepared. Parameters influencing the forming of cell microencapsules like voltage, push speed, electrode distance and syringe inner meter are analyzed and optimized. Adipose derived stem cells (ADSCs) microcapsules are prepared via the device. After one-week-long culture, ADSCs demonstrate high viability and significant proliferation.