鹅TLR7的分子克隆及生物信息学分析

oll like receptors (TLRs), which are the so-called pattern recognition receptors, mediate the host innate immune response and further adaptive immune. TLR7 is one of the TLR family that especially binds single-strand RNA (almost ssRNA virus or bacteria) and rapidly transmit the signaling to the downstream immune factor inducting self-defense. In this study, we successfully isolated the total RNA from Sichuan white goose and cloned the full length of goose TLR7 cDNA by RT-PCR and Rapid-amplification of cDNA ends (RACE). Finally, we found that goose TLR7 mRNA is 3900bp with a 1045 aa ORF. As a transmembrane protein we predicted through online software, TLR7 has a signal peptide sited in position 1 to 27, transmembrane domain is sited in position 836 to 860, and the homology and conserved Toll/IL-1 Receptor (TIR) domain is sited in position 884 to 1034. When compared the amino acid alignments of TLR7 form different animals, we found that the more affinity between two species showed the more identity in the sequences. The successful work for goose TLR7 cloning is helpful for the further homology and immunology study and also provides a base line to uncover the mechanism of poultry innate immune.