B-8大孔树脂吸附解吸中药复方多糖的研究

his study investigated compound immune enhancer of polysaccharides were extracted, and phenol hydrate-sulfuric for content determination,tested the absorption and desorption of Chinese medicine compounding polysaccharide with AB-8 macroporous resin.The polysaccharide was extracted by phenol-sulphoacid method,and with polysaccharide adsorption rate and desorption rate for index,to the AB - 8 macroporous resin adsorption and desorption conditions are optimized. Results showed that the sample liquid concentration 5.93 mg/mL, sample volume on the 1BV,sample speed 2 BV/h, elution agents ethanol concentration 50%, elution rate 3BV/h, elution agent volume for 3 BV ,the dynamic adsorption rate is 74.3%,and the dynamic desorption rate is 93.6%, which suggested that AB-8 macroporous resin can be used as purified polysaccharide material.