RNAi抑制内源性基因表达在肝脏疾病研究中的应用
RNAi Inhibits Endogenous Genes Expression in Hepatic Diseases
RNA干扰（RNA interference , RNAi）是由双链RNA所引起的能够序列特异性诱发同源基因转录后沉默的现象。这一现象广泛存在于各种生物体当中，如植物、线虫、果蝇、锥虫及哺乳动物细胞。近年来，人们利用RNAi技术，不仅成功地在体外实现了对病毒等外源性基因的抑制，而且也探索了在体外和动物体内对某些内源基因异常表达的抑制。本文报告了这一技术抑制内源性基因表达在肝脏疾病研究中的应用。
RNA interference ( RNAi ) is a process of post-transcriptional gene silencing ( PTGS ) initiated by double-stranded RNA ( dsRNA ) that is homologous in sequence to the silenced gene . This phenomenon occurs in a varieties of organisms including plants , Caenorhabditis elegant , Drosophila and trypanosomes , more recently in mammalian cells . RNAi has been considered as a mechanism of self-protection , not only fighting against the exogenous invading genetic elements such as virus but also inhibiting the abnormal expression of endogenous genes in animals. Here we report the developments of RNAi inhibiting endogenous genes effect in hepatic diseases.
张国英、赵中夫
基础医学分子生物学生物科学研究方法、生物科学研究技术
RNA干扰技术，内源基因，TNF-α，Fas，caspase-8
RNAi endogenous genes TNF-α， Fas，caspase-8
张国英,赵中夫.RNAi抑制内源性基因表达在肝脏疾病研究中的应用[EB/OL].(2004-05-03)[2025-08-10].http://www.paper.edu.cn/releasepaper/content/200405-13.点此复制
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