紫茎泽兰叶片水浸液对蚕豆的化感效应

Exotic invasive plants can alter ecosystem and displace the native plants by releasing allelochemicals to the surrounding environment by means of leaching, natural volatilization, root exudation and plant litter decomposition. In this study, Vicia faba seeds were treated with different concentrations of Eupatorium adenophorum leaf aqueous extracts. The effects of Eupatorium adenophorum leaf aqueous extracts on the micronucleus, chromosome aberration, apoptosis, chlorophyll and N content and photosynthetic physiological characteristics of Vicia faba were studied in this paper. The results were as follows: (1) the treatment of Eupatorium adenophorum leaf aqueous extracts significantly inhibited the root tip elongation and cell mitosis of Vicia faba, and induced chromosome aberration and cell micronucleus in the root tip cells of V. faba. With the increasing concentration of leaf water extracts, mitosis index significantly decreased and micronucleus rate of root tip cells significantly increased. In addition, the treatment of high concentration leaf aqueous extracts significantly affects apoptosis and necrosis of V . faba root tip cells. (2) the content of chlorophyll and N in leaves of V . faba decreased significantly, and the maximum net photosynthetic rate and biomass of V. faba seedlings were significantly decreased. In conclusion, Eupatorium adenophorum leaf aqueous extracts may cause the oxidative damage oftheroottipofViciafabaand theelongationoftheroot tip,andtheinhibitoryeffect ofleafwater extract shows a certain dose effect.At the same time, the damage and inhibition effect of Eupatorium adenophorum leaf aqueous extracts on the root tip of Vicia faba may affect the uptake of nitrogen, and thus have a significant negative effect on the photosynthetic physiological performance and biomass accumulation of broad bean seedlings. The results reveal the mechanism of Eupatorium adenophorum leaf aqueous extracts, and promote the understandingpotentialimpactofexoticinvasiveplantsonagriculturalproduction.