温阳散结法干预大鼠乳腺癌癌前病变对ki67 蛋白及mRNA表达的影响
Research About Worm-yang-disperse-plegm Method Inhibiting Effect on The Expression of Ki67 Protein And mRNA of Rats With Breast Precancerous
目的：研究阳和化岩汤为代表的温阳散结中药对大鼠乳腺癌癌前病变的抑制作用以及对ki67蛋白和mRNA表达的影响作用。方法：采用DMBA诱导建立大鼠乳腺癌癌前病变模型，9周后分组用药干预治疗4周，HE染色观察大鼠乳腺组织病理形态学表现，免疫组化法检测ki67蛋白表达的阳性率和灰度值，Q-PCR检测ki67mRNA表达。结果：各治疗组大鼠乳腺组织病理形态学表现较疾病模型组病变程度轻（P<0.05）；阳和化岩汤组癌前病变发生率为47.06%，浸润性癌发生率为29.41%，与其他治疗组比较，无统计学差异（P＞0.05）。ki67灰度值散结化痰汤组最低（101.18±21.73），三苯氧胺组最高（109.48±25.30），与阳和化岩汤组（106.28±23.74）比较，均无统计学差异（P＞0.05）。ki67阳性率平消片组最高（52.50%），散结化痰汤组最低（43.58%），与阳和化岩汤组（46.08%）比较，平消片组较高（P=0.0394），其他组无统计学差异（P＞0.05）。ki67mRNA表达量平消片组最高（F=0.92±0.30），三苯氧胺组最低（F=0.54±0.25），与阳和化岩汤组（F=0.69±0.32）比较，平消片组表达高（P=0.0462），其他组无统计学差异（P＞0.05）。结论：温阳散结法能够部分阻断逆转乳腺癌癌变，并具有抑制ki67蛋白和mRNA表达的作用，其作用与三苯氧胺接近，优于散结化痰法及平消片，适用于乳腺癌癌前病变的预防治疗。
Object: To research Worm-yang-disperse-plegm method delegated by Yanghehuayan Decoction inhibition effect on rats with precancerous lesions of breast carcinoma, and on the expression of Ki67 protein and mRNA. Methods: Rat mammary precancer models were established by DMBA, 9 weeks later, the rats were grouped and treated for 4 weeks separately, observing pathological morphology of breast tissue showed HE staining，detecting the expression positive rate and gray value of Ki67 by IHC, and expression of ki67mRNA by Q-PCR. Results: The gray value of Ki67: Sanjie-Decoction group was the lowest (101.18 ± 21.73), tamoxifen group was the highest (109.48 ± 25.30), compared with Yanghehuayan-Decoction group (106.28 ± 23.74), showed no significant difference (P > 0.05).The positive rate of Ki67: Pingxiao-tablets group was the highest (52.50%), Sanjie-Decoction group was the lowest (43.58%), compared with Yanghehuayan-Decoction, PingXiao-tablet group were higher (P=0.0394), and the other groups had no significant difference (P > 0.05).The expression of ki67mRNA: Pingxiao-tablets group was the highest (F=0.92 ± 0.30), tamoxifen group was the lowest (F=0.54 ± 0.25), compared with Yanghehuayan-Decoction group (F=0.69 ± 0.32), Pingxiao-tablets group were higher (P=0.0462), and the other groups had no significant difference (P > 0.05). Conclusion: Worm-yang-disperse-plegm method could prevent and reverse breast cancer carcinogenesis in a certain distant, inhibit Ki67 protein and mRNA expression. Its effect was similar to tomoxifen, superior to Sanjie-Decoction and Pingxiao-tablets, so this method should be suitable for the prevention and treatment of breast precancer.
陈翰翰、时光喜、李静蔚、刘晓菲、陈宏志、王世军
中医学肿瘤学基础医学
温阳散结法阳和化岩汤乳腺癌癌前病变ki67
Worm-yang-disperse-plegm methodYanghehuayan Decoctionbreast precancerous lesionki67
陈翰翰,时光喜,李静蔚,刘晓菲,陈宏志,王世军.温阳散结法干预大鼠乳腺癌癌前病变对ki67 蛋白及mRNA表达的影响[EB/OL].(2013-02-25)[2025-05-11].http://www.paper.edu.cn/releasepaper/content/201302-396.点此复制
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