辐射敏感和不敏感非小细胞肺癌microRNA表达谱的差异

Objective To investigate the different miRNA expression profile of radiotherapy sensitive and resistant patients of non-small cell lung cancer. Methods Thirty non-small cell lung cancer patients were divided into radiotherapy sensitive group and resistant group. Expression profile of miRNA in these two groups was detected by a microarray assay and the results were validated by quantitative RT-PCR. Results Comparing with resistant patients, five miRNA (miR-126, miR-let-7a, miR-495, miR-451, miR-128b) were significantly up-regulated and seven miRNA (miRNA-130a, miRNA-106b, miRNA-19b, miRNA-22, miRNA-15b, miRNA-17-5p, miRNA-21) were greatly down-regulated in radiotherapy sensitive group. Conclusion These twelve miRNA found in the present study may be correlated with the radiotherapy sensitivity of non-small cell lung cancer.