γδT细胞在沙眼衣原体呼吸道感染中的作用

Our research discussed the role of γδT cells in mice with Chlamydia trachomatis respiratory infection. WT mice and TCRδ-/- mice were intranasally inoculated with approproiate Chlamydia muridarum (Cm) to induce the Lung infection model. The percentage change of CD3+γδT cell in the lung was detected by flow cytometry technique to confirm the proliferation of γδT in different periods after the Cm infection. The burden of chlamydia in lung tissue was test by enzyme immunoassay. Lung sections were evaluated after staining with hematoxylin and eosin to score the degree of lung inflammation. The results show that γδT cells increased following infection of Cm and reached the peak on day 7. No apparent discrepancies were observed between WT and TCRδ-/- mice. however, lower weight and more severe pathologic alteration in lungs were observed in TCRδ-/- mice. From the above, γδT cells play an pivotal role in protective immunity during early stage of Cm infection.