根癌农杆菌介导的大麦茎尖转化研究

o develop a method for transformation of barley shoot apex through Agrobacterium-mediated strategy, two elite barley cultivars from China were used the transformation with their shoot apexes as explants. After selection on PPT media, transgenic lines resistant to herbicide were obtained. PCR identification showed that four transgenic lines derived from the cultivar E-damai9, and ten transgenic lines from the cultivar E-damai32122 contained expected transgenes, with a transformation efficiency of 0.59% (E-damai9) and 1.96% (E-damai32122), respectively. Southernblot analysis of T1 transgenic lines demonstrated that foreign genes were indeed integrated into the barley genome. From the analyzed transgenic lines, it is clear that all transgenic lines contained one copy per genome, and different lines displayed varied patterns of the transgenic integration. Thus barley shoot apex can be used for genetic transformation via Agrobacterium tumefaciens, and the current results provide useful information for exploitation of barley shoot apex as explants for genetic transformation.