Ribosome clustering and surface layer reorganization in the microsporidian host-invasion apparatus
Ribosome clustering and surface layer reorganization in the microsporidian host-invasion apparatus
Abstract During host cell invasion, microsporidian spores translocate their entire cytoplasmic content through a thin, hollow superstructure known as the polar tube. To achieve this, the polar tube transitions from a compact spring-like state inside the environmental spore to a long needle-like tube capable of long-range sporoplasm delivery. The unique mechanical properties of the building blocks of the polar tube allow for an explosive transition from compact to extended state and support the rapid cargo translocation process. The molecular and structural factors enabling this ultrafast process and the structural changes during cargo delivery are unknown. Here, we employ light microscopy and in situ cryo-electron tomography to visualize multiple ultrastructural states of the polar tube, allowing us to evaluate the kinetics of its germination and characterize the underlying morphological transitions. We describe a cargo-filled state with a unique ordered arrangement of microsporidian ribosomes, which cluster along the thin tube wall, and an empty post-translocation state with a reduced diameter but a thicker wall. Together with a proteomic analysis of endogenously affinity-purified polar tubes, our work provides comprehensive data on the infection apparatus of microsporidia and demonstrates that ribosomes are efficiently transported through polar tubes in a spiral-like parallel arrangement.
Carlson Lars-Anders、Ehrenbolger Kai、Jespersen Nathan、Barandun Jonas、Sharma Himanshu
Department of Medical Biochemistry and Biophysics, The Laboratory for Molecular Infection Medicine Sweden (MIMS), Wallenberg Centre for Molecular Medicine, Ume? Centre for Microbial Research (UCMR), Ume? UniversityDepartment of Molecular Biology, The Laboratory for Molecular Infection Medicine Sweden (MIMS), Ume? Centre for Microbial Research (UCMR), Science for Life Laboratory, Ume? University||Department of Medical Biochemistry and Biophysics, The Laboratory for Molecular Infection Medicine Sweden (MIMS), Wallenberg Centre for Molecular Medicine, Ume? Centre for Microbial Research (UCMR), Ume? UniversityDepartment of Molecular Biology, The Laboratory for Molecular Infection Medicine Sweden (MIMS), Ume? Centre for Microbial Research (UCMR), Science for Life Laboratory, Ume? UniversityDepartment of Molecular Biology, The Laboratory for Molecular Infection Medicine Sweden (MIMS), Ume? Centre for Microbial Research (UCMR), Science for Life Laboratory, Ume? UniversityDepartment of Molecular Biology, The Laboratory for Molecular Infection Medicine Sweden (MIMS), Ume? Centre for Microbial Research (UCMR), Science for Life Laboratory, Ume? University||Department of Medical Biochemistry and Biophysics, The Laboratory for Molecular Infection Medicine Sweden (MIMS), Wallenberg Centre for Molecular Medicine, Ume? Centre for Microbial Research (UCMR), Ume? University
微生物学细胞生物学分子生物学
MicrosporidiaPolar tubeCryo-electron tomographyRibosomePolar tube proteins
Carlson Lars-Anders,Ehrenbolger Kai,Jespersen Nathan,Barandun Jonas,Sharma Himanshu.Ribosome clustering and surface layer reorganization in the microsporidian host-invasion apparatus[EB/OL].(2025-03-28)[2025-06-27].https://www.biorxiv.org/content/10.1101/2023.05.31.543061.点此复制
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