马蹄多糖的提取工艺及其抗氧化性的研究

he water chestnut as raw material, the extraction conditions of polysaccharide were studied by single factor and orthogonal experiment, antioxidant activity on polysaccharide from water chestnut was detected respectively by Prussian blue method determination on reducing power and salicylic acid method determination on the hydroxyl radical scavenging ability. The results showed that extracting process of polysaccharidefrom water chestnut had microwave power of 600 W, 1:15 at the solid-liquid ratio, extracting time of 3 min, under these conditions, the extraction rate of polysaccharide could reach 8.12%, the polysaccharide purity could up to 83.2%. The polysaccharide from water chestnuthad strong reduction powerand scavenging ability to hydroxyl radicals, and the removal rate of hydroxyl radical reached 46.18% when the concentration of polysaccharide was 0.1 mg/mL.