转基因油菜GT73侧翼序列扩增及转化事件特异性检测方法研究

his paper describes an event-specific PCR method for qualitative of genetically modified canola GT73 base on the left integration junction characterized by inverse-PCR and semi-nested PCR. Sequence alignment between the T - DNA sequence and isolated junction fragments showed that a 2426 bp junction fragment of GT73 contained 2372bp of T - DNA sequence and 54 bp of canola genome DNA. Gt73 event-specific qualitative PCR method was established with the specific primers targeting the junction regions to produce a 522bp product. The event-specific qualitative PCR assay could distinguish GT73 from different genetically modified canola and showed 0. 05% limits of detection. The method developed in this study proved to be highly specific, accurate, sensitive, and suitable for GT73 event-specific detection.