禾谷镰刀菌随机插入突变体文库的构建

In this paper, the mutation library of Fusarium graminearum Y2021A inserted randomly by hph was constructed. The protoplast of strain Y2021A was prepared and the DNA fragments of hph was transformed to Y2021A to obtain the randomly inserted mutations.10163 mutations inserted by hph were totally obtained, 10125 of which considered as positive mutations could grow normally on PSA plate containing 100 μg/mL hygromycin B. Southern blot assay showed that the hph gene inserted different sites in the genome by one copy in five selected mutants. Five mutants whose pathogenicity significantly reduced were selected and they were the important materials for studying pathogenicity related genes. The mutant library is the precious resouce for studying pathogenicity mechanism and others of Fusarium graminearum.