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一种利用热可逆超分子凝胶低温冻存胰岛细胞的方法

Islets cryopreserved in themial reversible supramolecular gel system

中文摘要英文摘要

胰岛细胞的移植是治疗糖尿病的有效手段,但每次分离得到的活体细胞数量很难满足一次临床移植的需要,因此胰岛细胞需要通过低温冻存来达到长期储存的目的。本文利用凝胶因子在胰岛细胞悬浮液中自组装生成超分子凝胶低温冻存体系,该体系由于具有能限制冰晶生长的三维网络结构,从而有效降低了细胞的冻存损伤。另外,由于超分子凝胶具有热可逆性,在复温过程中能被去除,因此复温后的胰岛细胞的活性和功能与未经冻存的胰岛细胞相差甚小。

Islets transplantation is an important method for the treatment of diabetes, but it is difficult to preserve enough health islets for transplantation. Cryopreservation is the most common method to preserve cells for a long time. The gelator self-assembled to supramolecular gel cryopreservation system in the islets suspension. Cell damage can be reduced significant since the three-dimensional network structure can confine the ice crystal growth. In addition, because the supramolecular gel is thermal reversible, it can be easy removed in the thawing process. The thawed islets activities and functions are similar to fresh islets.

陈熹、曾杰、张丽、陈万煜

基础医学生物科学研究方法、生物科学研究技术细胞生物学

胰岛细胞超分子凝胶低温冻存

Isletssupramolecular gelcryopreservation

陈熹,曾杰,张丽,陈万煜.一种利用热可逆超分子凝胶低温冻存胰岛细胞的方法[EB/OL].(2015-12-11)[2025-08-11].http://www.paper.edu.cn/releasepaper/content/201512-696.点此复制

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