Kidney osteoclast factors and matrix metalloproteinase expression in a mice model of diet-induced obesity and diabetes
Kidney osteoclast factors and matrix metalloproteinase expression in a mice model of diet-induced obesity and diabetes
ABSTRACT The role of RANKL/RANK/OPG system on bone remodeling is well known, and there is evidence that it is also important to cardiovascular and kidney pathology, although the underlying mechanisms are not elucidated so far. Thus, we investigated in a mice model of diet-induced obesity and diabetes if renal histopathological changes are associated with the expression of RANKL/RANK/OPG system and matrix metalloproteinases (MMPs). Three months old C57BL/6 mice were fed with control (AIN93M) or high-fat high sucrose (HFHS) diets for 21 weeks (CEUA/UFF #647/15). The HFHS group showed weight gain (+35%, P=0.0001), increased epididymal, inguinal and retroperitoneal fat pad weight (+121 %, P = 0.0006; +287 %, P = 0.0007 and; +286 %, P < 0.0001, respectively), and hyperglycemia (+43%, P=0.02). The kidney of some HFHS fed mice displayed mononuclear inflammatory cell infiltrate (40%), perivascular fibrosis (20%), and focal tubule mineralization (20%). Glomeruli hypertrophy was not detected. Unexpectedly, OPG, RANK, MMP-2 and MMP-9 expression was not altered in HFHS groups (Western blot analysis). In conclusion, the expression of RANKL/RANK/OPG system proteins and MMPs was not influenced by diet-induced obesity and diabetes in the kidney of male C57BL/6 mice, although some adverse histopathological remodeling is noticed in the renal tissue.
Martins-Gregorio Bianca、Paes-de-Almeida Elan Cardozo、de Souza Mendon?a Leonardo、de Souza Azevedo Rebeca、Fernandes-Santos Caroline、dos Santos-Macedo Francine
Unidade de Pesquisa Urogenital, Universidade do Estado do Rio de JaneiroLaborat¨?rio Multiusuario de Pesquisa Biomedica, Departamento de Ciencias Basicas, Universidade Federal Fluminense, Instituto de Saude de Nova FriburgoLaborat¨?rio Multiusuario de Pesquisa Biomedica, Departamento de Ciencias Basicas, Universidade Federal Fluminense, Instituto de Saude de Nova FriburgoLaborat¨?rio de Patologia Oral, Departamento de Formacao Especifica, Universidade Federal Fluminense, Instituto de Saude de Nova FriburgoLaborat¨?rio Multiusuario de Pesquisa Biomedica, Departamento de Ciencias Basicas, Universidade Federal Fluminense, Instituto de Saude de Nova FriburgoLaborat¨?rio Multiusuario de Pesquisa Biomedica, Departamento de Ciencias Basicas, Universidade Federal Fluminense, Instituto de Saude de Nova Friburgo
基础医学生物科学研究方法、生物科学研究技术
RANKOPGobesitydiabeteskidney
Martins-Gregorio Bianca,Paes-de-Almeida Elan Cardozo,de Souza Mendon?a Leonardo,de Souza Azevedo Rebeca,Fernandes-Santos Caroline,dos Santos-Macedo Francine.Kidney osteoclast factors and matrix metalloproteinase expression in a mice model of diet-induced obesity and diabetes[EB/OL].(2025-03-28)[2025-04-26].https://www.biorxiv.org/content/10.1101/615716.点此复制
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