HPLC法测定单面针药材中白鲜碱含量

Objective：An analysis method using high performance liquid chromatographic for determining Dictamnine in Zanthoxylum Dissitum Hemsl had been successfully established in this paper. Method：In this experiment, Dictamnine was obtained by ultrasonic extraction and separated on SinoChrom ODS-BP C18 column (4.6 mm×250 mm, 5 μm) and the mobile phase of it was mixture liquid of acetonitrile and 0.01 mol/L NaH2PO3 (50:50, v/v). The flowing rate of the mobile phase was 0.8 ml/min, and the wavelength for detecting Dictamnine was 236 nm. Results：As the results indicated, when the injection volume of the Dictamnine ranged from 0.0015 to 0.2 μg, a significant linear relationship was showed between? the peak area and the mass concentration (R2＝0.9995). Further, the average recovery rate of Dictamnine was 98.8%, and the relative standard deviation (RSD) was 3.06% (n=6). Conclusion：The established analysis method possessed accuracy, reliability and high repeatability, which made it a suitable detection method for determination of Dictamnine in Zanthoxylum Dissitum Hemsl of different origin place and medicinal parts.