牛乳铁蛋白对仔猪抗菌肽Protegrin-1 mRNA表达的影响

his experiment was conducted to study the effect of bovine lactoferrin (bLF) on antimicrobial peptide Protegrin-1 mRNA expression. In vivo，ninety weaning piglets (Duroc×Landrace×Large white) at an average weight of 9.70 kg were divided into three groups randomly, 30 piglets each group. The dietary treatments were :(1) basal diet(Control), (2) basal diet + 0.125% bLF, and (3) 0.25% bLF. In vitro, bone marrow cells were separated and cultured with bLF at the final concentration of 10, 100, and 1000&micro;g/ml respectively for 3, 6, 12 and 24h. The control cells were incubated with culture medium. Taking 18S rRNA as inner control, the effect of bLF on Protegrin-1 mRNA expression was detected by semi-quantitative RT-PCR. The results showed that the expression levels of Protegrin-1 increased by 212.6%（P<0.05）and 106.3%（P>0.05） when the basal diet was added 0.125% bLF and 0.25% bLF respectively. After the bone marrow cells were treated with 10, 100, and1000&micro;g/ml bLF for 3 and 6h, the expression levels increased at 3 and 6h but decreased at 12 and 24h. It suggested that antimicrobial peptide gene expression of Protegrin-1 could be regulated by bLF.