甲状腺乳头状癌中丙酮酸脱氢酶激酶和乳酸脱氢酶的表达及与BRAF关系的研究

im To explore the expression pattern of pyruvate dehydrogenase kinase-1 (PDK1), PDK2 and lactate dehydrogenase-A (LDH-A) in papillary thyroid carcinoma (PTC), and to explore the relationship of the expression of these key enzymes and BRAF mutation in PTC. Method The expression of PDK1, PDK2 and LDH-A were examined in 150 PTC tissues and 100 normal thyroid tissues using immunohistochemical staining. BRAF mutation was examined in 79 above PTC tissues using PCR sequencing. Results The positive rate of PDK1, PDK2 and LDHA in PTC tissues was 76%, 81% and 95%, respectively. The positive rate in normal thyroid tissues was 20%, 16% and 30%, respectively, which were lower than those in PTC tissues (P<0.001). The expression scores of PDK1, PDK2 and LDHA were higher in PTC than those in normal thyroid tissues (P<0.001). The positive expression of PDK1 was associated with BRAF mutation in PTC tissues (P<0.05). The rate of lymphatic metastasis was higher in group with positive PDK1 than that in group with negative PDK1 (P<0.05). Conclusion The overexpression of PDK1 may contribute to the growth of PTC cells through activation of glycolysis. BRAF mutation may change the expression of the key enzymes involving in glycolytic pathway and promote the development of PTC.