端粒酶重建皮肤成纤维细胞迁移研究

ransplantation of myoblasts is a potential treatment for Duchenne muscular dystrophy (DMD). However, this treatment is currently not feasible because of myoblasts’ low proliferation and migration capacity. Since myoblasts can be induced from fibroblasts, we have reconstituted fibroblasts with telomerase to enhance the lifespan of fibroblasts, and studied the migration of the telomerase reconstituted fibroblasts. We found that when stimulated with a chemokine, CXCL12, the rate of migration was significantly higher in fibroblasts with telomerase reconstitution than that in fibroblasts without. Furthermore, the CXCL12 receptor, CXCR4, and multiple down-stream factors (Rho family members), were upregulated in the telomerase reconstituted fibroblasts. We concluded for the first time that telomerase reconstitution enhances fibroblast migration through activation of CXCL12/CXCR4 axis and Rho family. The finding that fibroblasts with telomerase reconstitution have enhanced migration may have broad implications, especially when these fibroblasts were used to treat DMD by inducing them into myoblasts.