dTe量子点荧光探针与DNA的相互作用研究

In this paper, 3-MPA (3-mercaptopropionic acid) stabilized CdTe quantum dots (QDs) were prepared by direct aqueous synthesis method. The interactions of QDs with DNA were studied through two steps. First, by adding excessive Cd2+ under basic conditions, the prepared CdTe QDs was activated and thus cationic CdTe QDs was obtained. The effects of pH, activation time and Cd2+ concentration on activation efficiency were also studied in details. Basing on the electrostatic interactions between DNA and cationic Cd2+ enriched QDs, the fluorescence of QDs was quenched upon addition of DNA, and the quenched fluorescence intensity was in good linear relationship with the concentration of DNA (R=0.99198). This method developed here shown great potential for DNA detection in complex samples.