维生素C通过调控成脂相关基因的表达影响3T3-L1前脂肪细胞成脂分化

he effect of vitamin C (Vit.C) on preadipocyte differentiation is not a result currently. In the process of inducing the osteogenic differentiation of animal stem cells in vitro, Vit.C is an essential inducer. Therefore, the molecular mechanism of role of Vit.C on adipocytes differentiation need to be evaluate. Here, we use 3T3-L1 preadipocyte cell lines to explore the optimal concentration of Vit.C and the changes of the time sequence characteristics of the PPAR gamma 2, CEBPs, SREBP-1 and adiponectin transcription and translation of the fat related genes in the differentiation process. The results showed that with the increasing of Vit.C concentration, the lipid content of the cells increased, the content of Vit.C 250 and 500μM was the highest, and there was no significant difference between the two groups. The mechanism may be through the regulation of transcription factor PPARγ2 and C/EBPαand lipid metabolism related gene SREBP-1 sequence regulation, promoting the expression of the fat gene, thereby promoting the differentiation of fat cells.