神经生长因子及其受体在蛋鸡等级前卵泡上的表达

In order to investigate the expression level of nerve growth factor(NGF)and its receptors in the prehierarchical follicle of hens,2 groups of 83 and 230 days old laying hens were examined by immunohistochemistry and fluorescence quantitative PCR analyses,each group contain 5 birds. The results demonstrated that NGF and its two receptors were not detected in the ovary of 83-days old laying hens, but expressed in thecal cells and granulosa cells of pre- hierarchical follicles of 230-days old laying hens.Expression of NGF and its receptors TrkA and p75 in small yellow follicle (SYF) of 230 days were significantly higher than that in small white follicle(SWF) and large white follicle(LWF).Furthermore ,serum levels of estrogen and/or progesterone were significantly higher in 230 days than that in 83 days.Taken together, these results indicated that NGF may play a role in the growth, development and ovulation of hierarchical follicles,and it may also affect the laying rate in different laying periods..