一组小麦秸秆好氧分解菌系构建及组成多样性

microbial community with the stable ability of effectively decomposing wheat stalks was constructed at room temperature under aerobic conditions by the method of restricted cultivation.The degradation ability of microbial community shows that when microbial community was performed in 100mL improved CMC liquid medium with depth 2cm and diameter 9cm,microbial community kept higher degradation efficiency with capable of decomposing straws of 66.1% during the initial six days.By the tenth day of decomposition,the degradation ratio was slowly up to 77.0%.For the components of wheat stalk,cellulose lost 0.78g,hemicellulose lost 0.16g and lignin lost 0.24g.The composition diversity of microbial community shows that 13 genuses of microorganisms were identified by the technologies of clone labrary and isalation of pure strain and the dominant genuses were Hydrogenophaga,Pseudomonas,Bacteroides and Clostridium taking up 78% in one hundred of positive clones.Phylogenetic dendrogram indicates that the relations among clones and isolated strains and their closest relatives presented certain distances;both Isolated 7(FJ439527) and Clone 86(EU834839) had a near relation with the novel species of pseudomonas xiamenensis.