孕期镉暴露对子代发育及海马CA1区组织结构的影响

Objective: The purpose of this study is to observe the effect of cadmium expose during pregnancy on offspring growth and the morphology of CA1 district of hippocampus. Method :We established the animal model of cadmium exposure by adding CdCl2 to the drinking water of pregnant mice and divided the offspring into 2 groups: control group and cadmium exposure group. And then we applied graphite furnace atomic absorption spectrometry to determine the concentration of serum cadmium and its exposure condition during 3 weeks and 5 weeks after delivery. HE staining was used to observe the morphological changes in neuronal CA1 district of hippocampus during 5 weeks after delivery. Result: the body weight of offspring from exposure group was lower than that in control group during 3 and 5 weeks after delivery. The result of graphite furnace atomic absorption spectrometry had showed that the concentration of serum cadmium was increased significantly compared with that in the control group (P<0.05). HE staining showed that the neuronal of hippocampus from control group were arranged neatly and well-distributed, while the amount of neuronal cells were decreased and neuronal cell distributed disorderly and pathologically changed obviously in cadmium exposure group. Conclusion: the low-dose cadmium exposure during pregnancy would result in the accumulation of cadmium in offspring of mice, and show a certain influence on development of growth and morphology of CA1 district of hippocampus.