RPV6在蛋鸡不同肠段和肾脏中的定位表达

Object and Methods: Immunohistochemical analysis was used to investegate the TRPV6 localization and expression in different intestinal segments and kidney in laying hens during peak laying. In addition, changes of TRPV6 concentration among these tissues were obtained by the western-blot analysis. Results: TRPV6 was localized to the brush-border membranes of the duodenum, jejunum, ileum, cecum and rectum. Expression was weaker in the rectum and little or no expression was found in crypt and goblet cells. In the kidney, distinct immunopositive staining for TRPV6 was detected at the apical domain of the proximal convoluted tubules (PCT), distal convoluted tubules (DCT) and medullary connecting tubules (CNT). Furthermore, western-blot analysis indicated that the greatest amount of TRPV6 was expressed in the duodenum and the least in the rectum, expressing intermediate levels were in the other segments. Moreover, the kidney presented lower TRPV6 protein levels compared to duodenum. Conclusion：the epithelial Ca2+ channel TRPV6 is strongly expressed in the apical cells of the entire intestine and the renal tubules, suggesting that active Ca2+ transcellular transport plays a crucial role in dietary calcium (re)absorption in laying hens.