碱性果胶酶生产菌株的筛选和果胶酶系分析

Pectinase is a heterogeneous group of related enzymes that hydrolyze the pectic substances. In this paper, seven strains producing high activity of alkaline pectate lyase were screened from Tianjin alkaline soil and then the classification and activities of pectinase from all these stains were detected. The results showed that seven stains had enzyme activity of pectinesterase and pectate lyase, but stains 410,418 and 601 produced protopectinase which activity reached 0.341u/mL、0.51u/mL、0.28u/mL separately. Bacillus sp.410 and 418 showed Pectin hydrolysis enzymes activity of 0.035u/mL and 0.363u/mL.When texted by pectin with 60% esterification, only Bacillus sp.520 exhibited hydrolyses activity. All these shows that the pectinase diversity is abundant in different strains. Analysis of pectinase system can provide fundamental basis for the study of application of wild strain, construction and evaluation of engineering bacteria.