番茄花柄脱落区多聚半乳糖醛酸酶的提取与纯化

Polygalaturonase is an important hydrolase during abscission process. In this experiment，the pedicels explants of tomato in vitro treated with 20μL•L-1 ethylene were used to extract and purify the polygalacturonase related to abscission. The extraction and purification system as follows: 50mmol • L-1 acetate buffer (pH = 5.5) for the extract buffer by adding 0.1 mol•L-1 NaCl and 1mmol•L-1 DTT; the enzyme extracts then concentrated and purified by Sephadex G-75 chromatography gel with the optimum condition of 0.2 mL•min-1 flow rate and 3.5mL sample volume; then the part with high polygalacturonase activity was concentrated and CM Sepharose CL-6B ion exchange chromatography was used with 0.3 mL•min-1 flow rate and elute pH5.5. The result showed that Sephadex G-75 chromatography gel and CM Sepharose CL-6B ion exchange chromatography are the availability methods to purification and characterization of a polygalacturonase from tomato pedicel. The experimental laid the reference for the study of character and activity regulation of polygalacturonase related to abscission