柑橘丛枝菌根真菌的分子鉴定
Molecular identification of arbuscular mycorrhizal fungi in citrus
柑橘根毛稀少或缺乏，对养分的吸收主要通过与丛枝菌根真菌（AMF）形成共生来完成。然而，柑橘上的菌根真菌资源尚未得到系统的鉴定。本实验以华中农业大学柑橘果园温州蜜柑枳砧根系样品及其根际土壤为材料，通过对根系样品DNA扩增菌根真菌18S SSU rRNA区段进行测序，获得120个来自AMF的序列。通过与MAARJAM数据库比对，获得92个有效序列，共鉴定到2个属（Glomus和Paraglomus）的8个种，其中Glomus属的Afrothismia foertheriana symbiont占总序列数的85%。通过对土壤中的47个单孢子形态学观察和分子鉴定，获得5个属的7个种，其中Ambispora属的Ambisporaceae Ambispora leptoticha占总孢子数的75%。根系样品AM真菌的DNA测序和土壤AMF孢子鉴定得到的优势AM菌种属分别为Glomus属和Ambispora属。本实验初步鉴定出了华中农业大学温州蜜柑枳砧根系及土壤中的AMF优势属，为柑橘优势菌种的的筛选和纯化打下了基础。
itrus roots have short or rare root hairs, and thus they dependent on Arbuscular Mycorrhizal Fungal (AMF) for mineral nutrient uptake. However, the AMF community in citrus roots was still unknown. In this study, we investigated the AMF community structures in citrus roots from the orchard located in Huazhong Agricultural University. Through clone library sequencing, a total of 92 effective sequences from two genera were obtained. It is notable that 85% of the sequences were belonged to Afrothismia foertheriana symbiont (speices). In addition, a total of 47 spores were isolated from soil for morphology observation and molecular identification. Among these spores, 75% of them were belonged to Ambisporaceae Ambispora leptoticha (species). The results indicated that the dominant fungi in citrus roots and soil respectively were belonged to Glomus and Ambispora, which would provide a solid basis for the purification of beneficial AMF species for citrus industry.
王敏、潘志勇、宋放
分子生物学植物学微生物学
柑橘丛枝菌根真菌优势菌种
itrusarbuscular mycorrhizal fungidominant fungi
王敏,潘志勇,宋放.柑橘丛枝菌根真菌的分子鉴定[EB/OL].(2014-12-04)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/201412-119.点此复制
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